Shameik Moore
Miles Morales (voice)
Hailee Steinfeld
Gwen Stacy (voice)
Brian Tyree Henry
Jeff Morales (voice)
Luna Lauren Velez
Rio Morales (voice)
Jake Johnson
Peter B. Parker (voice)
Oscar Isaac
Miguel O'Hara (voice)
Jason Schwartzman
Spot (voice)
Issa Rae
Jessica Drew (voice)
Daniel Kaluuya
Hobie Brown (voice)
Karan Soni
Pavitr Prabhakar (voice)
Shea Whigham
George Stacy (voice)
Greta Lee
LYLA (voice)
Mahershala Ali
Uncle Aaron (voice)
Amandla Stenberg
Margo Kess: Spider-Byte (voice)
Jharrel Jerome
Miles G. Morales (voice)
Andy Samberg
Ben Reilly (voice)
Jack Quaid
Peter Parker (voice)
Rachel Dratch
Ms. Weber (voice)
Ziggy Marley
Lenny (voice)
Jorma Taccone
Adriano Tumino / '67 (voice)
J.K. Simmons
J. Jonah Jameson (voice)
Donald Glover
Aaron Davis
Elizabeth Perkins
May / Quippy Spider-Person (voice)
Kathryn Hahn
Doc Ock (voice)
Ayo Edebiri
Glory (voice)